基因A型鸭甲肝病毒弱毒株免疫雏鸭的8种细胞因子mRNA变化分析

doi:10.11843/j.issn.0366-6964.2016.06.017

Abstract

Abstract:

In order to explore the roles of cytokines responding to attenuated duck hepatitis A virus genotype A (DHAV-A) vaccine immunization，the effects of attenuated DHAV-A MY strain on cytokine transcription in ducklings were analyzed.The mRNA transcription levels of 8 cytokines，such as IFN-α，IFN-β，IFN-γ and IL-1β，IL-2，IL-6，IL-8，and TNF-α，were determined by RRT-PCR in liver，spleen and brain of ducklings at different time points of 0.25 0.5，1，2，3 and 5 days post attenuated DHAV-A MY strain injecting 1-day-old SPF ducklings，and the virus loads of DHAV-A MY strain in liver，spleen and brain were also detected by RRT-PCR.The results indicated that the virus load reached the highest level in liver and brain at 2 d time point，and at 3 d time point in spleen post attenuated DHAV-A MY strain injection.The mRNA transcription levels of 8 cytokines in liver，spleen and brain up-regulated very significantly at the corresponding time points when the virus load reached the highest level post DHAV-A MY strain injection (P<0.01).The results indicated that attenuated DHAV-A MY strain could stimulate the transcription of anti-viral cytokines IFN-α，IFN-β，IFN-γ and TNF-α，as well as proinflammatory cytokines IL-1β，IL-2，IL-6 and IL-8，our results enrich the immunization mechanism of DHAV-A MY strain.

CLC Number:

S855.3

ZHANG Huan-rong，ZHANG Bing，WANG Dong，YUE Hua. mRNA Change Analysis of Eight Cytokines in Ducklings Stimulated by Attenuated Duck Hepatitis A Virus Genotype A MY Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1215-1221.

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mRNA Change Analysis of Eight Cytokines in Ducklings Stimulated by Attenuated Duck Hepatitis A Virus Genotype A MY Strain

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